Pengaruh Pemberian Ekstrak Daun Sirsak terhadap Kadar SGPT dan SGOT Tikus yang Di Induksi Obat Anti Tuberkulosis Isoniazid dan Rifampisin
Abstract
Background: The use of the antituberculosis drugs isoniazid and rifampin triggers hepatotoxicity. Soursop leaves have hepatoprotective effect because they contain antioxidant compounds that can help inhibit and prevent oxidative damage to the liver. This study aims to determine the effect of soursop leaf extract on SGPT and SGOT levels in rats induced by isoniazid and rifampicin.
Methods: This experimental laboratory study used the post-test-only control group design. This study used 25
wistar rats divided into five groups and given a 14-day treatment. Group 1 (negative control) was assigned isoniazidand rifampicin without administering soursop leaf extract. Group 2 (positive control) was given isoniazid and rifampicin by administering hepatoprotector compound. Group 3, 4, and 5 were given isoniazid and rifampicin for 1 hour, and continued with the administration of soursop leaf extract with a dose of 36 mg/day, 72 mg/day, and144 mg/day respectively. The dose of the isoniazid used was 189 mg/day, while the rifampicin dose was 252mg/day. Rat termination was carried out on the 15th day. Blood samples to measure SGPT and SGOT levels were taken through intracardial.
Results: The results showed that there was a significant difference in SGPT and SGOT levels between the
treatment group and negative control at doses of 72 mg/kg body weight/day and 144 mg/kg body weight/day. There was no significant difference between SGPT levels in the treatment group of dose of 144 mg/kg/day and the positive control group. There were significant differences in the SGOT levels of the treatment groups and the positive control group.
Conclusion: Administration of soursop leaf extract can lower SGPT and SGOT levels in rat induced with isoniazid
and rifampicin. The hepatoprotective effect of soursop leaves was obtained at a dose of 144 mg/kg body
weight/day.
Keywords: soursop leaf, isoniazid, rifampicin, SGPT, SGOT
References
2. T Tiberi S, Scardigli A, Centis R, D'Ambrosio L, Muñoz-Torrico M, SalazarLezama MÁ, Spanevello A, Visca D, Zumla A, Migliori GB, Caminero Luna JA. Classifying new anti-tuberculosis drugs: rationale and future perspectives. Int J Infect Dis. 2017 Mar;56:181-184.
3. Guyton AC, Hall JE. Buku Ajar Fisiologi Kedokteran. 11th ed. Jakarta: EGC; 2014. 859 p.
4. Ningsih ASW, Ramadhan AM, Rahmawati D. Kajian literatur pengobatan tuberkulosis paru dan efek samping obat antituberkulosis di Indonesia. 15thProc. Mul. Pharm. Conf. 2022. https://doi.org/10.25026/mpc.v15i1.647
5. Alshehri MM, Amjad MW, Mudawi MME. Drugs-Inducing Hepatotoxicity. Asian J. Pharm. Res. Health Care. 2020;12(3):148-156.
6. Kementrian Kesehatan RI. Strategi nasional penanggulangan tuberkulosis di Indonesia 2020-2024. 2020;135.
7. Parapaga VFS, Durry MF, Lintong PM. Efek pemberian ekstrak daun sirsak (Annona muricata L.) terhadap gambaran histopatologik hati tikus wistar (Rattus Norvegicus) yang diinduksi rifampisin. J e-Biomedik. 2018;6(2):195–9.
8. Amelia N. Efek hepatoprotektor ekstrak daun teh hijau (Camellia sinensis) pada tikus putih (Rattus novergicus) yang diinduksi isoniazid [skripsi]. Universitas Sebelas Maret. 2008.
9. Rahim A. Aktivitas daun cabe rawit (Capsicum frutescens L.) sebagai hepatoprotektor. Pharmed. 2019;2(1):7-13
10. Tuna MR. Uji daya hambat ekstrak daun sirsak (Annona muricata L.) terhadap pertumbuhan Staphylococcus aureus secara in vitro. PHA [Internet]. 2015 Nov. 9 [cited 2023 Aug. 11];4(4). Available from: https://ejournal.unsrat.ac.id/v3/index.php/pharmacon/article/view/10194
11. Wahyudi AD. Soedarsono. Farmakogenomik hepatotoksisitas obat anti tuberkulosis. Jurnal Respirasi (JR). 2015;1(3):103-108
12. Marinda FD. Hepatoprotective effect of curcumin in chronic hepatitis. J Majority. 2014;3(7):103-108
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
